Device

Part:BBa_K1979010:Design

Designed by: MA Xinyi   Group: iGEM16_SDSZ_China   (2016-08-25)


Promoter (BBa_J23101)+PBP5+T7 terminator


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 7
    Illegal NheI site found at 30
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 1045
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 359
    Illegal AgeI site found at 961
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

We added the histidine tag for affinity purification to harvest the proteins.


Source

The PBP5 and terminator sequences are retrieved form GenBank, and the promoter sequence comes from the part BBa_J23101.

References